PLGA Nanoparticles - Double Emulsion - Custom Cargo Loading

Description
Our double-emulsion poly (lactic-co-glycolic acid) (PLGA) nanoparticles form nanostructures with a hydrophillic core surrounded by a hydrophobic shell, which in turn is surrounded by a hydrophillic outer layer. This allows for loading of hydrophillic cargo, such as proteins, nucleic acids, or small moleules, inside the nanoparticle core.
Dye molecules can be encapsulated inside the hydrophobic core. PLGA surface contains COOH groups that can be used for EDC/NHS based bioconjugation. Optional surface modifications include methoxy PEG (improves in vivo blood half-life) and PEG-Azide (can be used to covalently attach biomolecules by click chemistry)
 
Features
Made with FDA approved biocompatible and biodegradable PLGA polymer.
Conjugation-ready for covalent attachment of biomolecules.
High degree of monodispersity.
Cab be loaded with hydrophillic cargo, such as proteins, nucliec acids, small molecules.
Labeled with a fluorescent dye.
(Optional) Incorporation of Methoxy-PEG minimizes non-specific protein adsorption. This increase nanoparticle in-vivo blood half-life.
(Optional) Incorporation of Azide-PEG allows conjugation of proteins and small molecules by click chemistry.
 
Applications
In vitro and in vivo delivery of proteins, nucliec acids and small molecules.
Fluorescence labeled nanoparticles for in vitro and in-vivo targeting.
Fluorescence imaging for in vitro and in vivo applications.
Fluorescence probes for flowcytometry analysis.
In vitro and in vivo delivery of proteins and small molecules.
 
NOTE: The customer will need to provide the cargo to be loaded into the PLGA nanoparticles. The loading takes place during the nanoparticle synthesis.