Protein A, Fluorescent Dye Labeled
Recombinant Protein A that has been covalently labeled with a fluorescent dye.
The following fluorescence dye labels are available:
- AFDye 488
- AFDye 555
- AFDye 647
- IR750
- NOTE 1: AFDye 488 (similar to FITC), AFDye 555 (similar to Cy3), AFDye 647 (similar to Cy5) are analogs of AlexaFluor® 488, AlexaFluor® 555, and AlexaFluor® 647 dyes, respectively.
- NOTE 2: IR750 dye is ithe analog of the IRDye® 750 dye.
Protein A is a cell wall component produced by a number of strains of Staphylococcus aureus. It consists of a single polypeptide chain with few or no carbohydrate. Recombinant Protein A is produced in E. coli and in the same manner as native Protein A. The Protein A molecule contains four high-affinity binding sites that can interact with the Fc region from IgG of a number of species. Optimal binding occurs at pH 8.2, but binding is also effective at physiological conditions (pH 7.0 to 7.6).
Applications
Luna Nanotech Protein A is provided for research laboratory or bioprocessing purposes only. Not for use in diagnostic or theraputic human or animal applications.
Formulation
Lyophilized powder
Storage
Store at 4°C if entire vial with lyophilized product will be used within 5 weeks. Store, frozen at -20°C . After reconstitution in distilled water or buffer, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Ig binding strength of protein A
Ig Species / Subtype
Affinity for Protein A
Human IgG
+++
Human IgG1
+++
Human IgG2
+++
Human IgG3
+
Human IgG4
+++
Human IgM
+
Human IgD
-
Human IgA1
+
Human IgA2
+
Human Fab
+
Human scFv
+
Mouse IgG
+++
Mouse IgM
-
Mouse IgG1
+
Mouse IgG2a
+++
Mouse IgG2b
+++
Mouse IgG3
+++
Rat IgG
+
Rat IgG1
+
Rat IgG2a
-
Rat IgG2b
-
Rat IgG2c
+++
Rabbit IgG
+++
Goat IgG
+
Sheep IgG
+
Guinea pig IgG
+++
Hamster IgG
++
Donkey IgG
++
Pig IgG
+++
Dog IgG
+++
Cat IgG
+++
Chicken IgG
-
Cow IgG
+
Horse IgG
+
Product Data Sheet