Protein A/G, Fluorescent Dye Labeled

Recombinant Protein A/G that has been covalently labeled with a fluorescent dye.
 
The following fluorescence dye labels are available:
AFDye 488
AFDye 555
AFDye 647
IR750
 
NOTE 1: AFDye 488 (similar to FITC), AFDye 555 (similar to Cy3), AFDye 647 (similar to Cy5) are analogs of AlexaFluor® 488, AlexaFluor® 555, and AlexaFluor® 647 dyes, respectively.
NOTE 2: IR750 dye is ithe analog of the IRDye® 750 dye.
 
Gene fusion of the Fc-binding domains of Protein A and Protein G results in production of a chimeric Protein A/G with broader binding compared to Protein A or Protein G alone. During fusion, the Protein G gene sequence coding for the serum albumin-binding site is removed to minimize non-specific interactions. The extended Fc-binding properties of Protein A/G make it a popular tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and less strongly to IgD. Protein A/G does not bind to mouse IgA or IgM. Protein A/G is an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from other serum proteins. Individual subclasses of mouse monoclonal antibodies are more likely to have strong affinity to Protein A/G compared to Protein A or Protein G.
 
Our recombinant Protein A/G is a fusion protein that comprises of a total of 7 IgG-binding domains EDABC-C2C3. This fusion protein combines the active Fc-binding domains of Protein A and Protein G that are contained in the recombinant sequence. The comprehensive Fc-binding properties of Protein A/G fusion make it a standard tool in the study and purification of immunoglobulins. Protein A/G fusion is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from other serum proteins.
 
Applications
Luna Nanotech Protein A/G is provided for research laboratory or bioprocessing purposes only. Not for use in diagnostic or theraputic human or animal applications.
 
Formulation
Lyophilized powder
 
Storage
Store at 4°C if entire vial with lyophilized product will be used within 5 weeks. Store, frozen at -20°C . After reconstitution in distilled water or buffer, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.