PuroSPIN-96™ Total RNA Purification Plus Kit

The PuroSPIN-96™ Total RNA Purification Kit is designed to purify total RNA from bacteria, mammalian cells, yeast, or animal tissue. It greatly simplifies the extraction process by incorporating a convenient and easy-to-use extraction technology based on nucleic acids binding to 96-well silica membrane purification plates.
In comparison to our standard PuroSPIN-96™ Total RNA Purification Kit, the Plus kit adds an additional filtration step that removes the insoluble and non-digested fraction as well as insuring elimination of genomic DNA from the lysate before loading it onto the silica spin column. This prevents contaminating materials from binding to and blocking parts of the silica membrane, leading to an increased RNA yield and higher purity of the final product. This is particularly benficial when workign with large samples that contain a lot of starting material that needs to be digested.
In addition the kit includes on-column DNase I digestion step to further ensure removal of any contaminating DNA. 
The kit can also be used to perform RNA cleanup after DNase I or other enzymatic treatments. High quality RNA extracted with the PuroSPIN-96™ Total RNA Purification Plus Kit can be used for a range of downstream applications, such as reverse-transcription PCR or qPCR, northern blotting, microarray analysis.
 
Features:
Extract total RNA from a variety of biological samples, with specialized protocols for each sample type
High throughput sample processing using 96-well silica membrane purification plates
Simple extraction process with no need for phenol or chloroform
Includes on-column DNase I digestion step to remove contaminating DNA
High quality extracted RNA can be used for a range of downstream applications such as cDNA library construction, microarray analyses, Northern blotting, qPCR, and RT-qPCR
 
SKU List: NKP251-384