PuroSPIN™ Endo-Free Plasmid Miniprep Kit #NKE111-50, #NKE111-250

PuroSPIN™ Endo-Free Plasmid Miniprep Kit is designed to provide simple and easy-to-use method for extraction and purification of plasmid DNA from cultured bacterial cells. The purification technology is based on plasmid binding to silica spin-columns, which allows efficient removal of genomic DNA, protein, salt and other contaminants. Following the lysis steps, endotoxins are removed using the Endotoxin Removal Buffer ERB.
 
The PuroSPIN™ Endo-Free Plasmid Miniprep Kit can extract up to 15 µg of plasmid DNA with endotoxin levels <0.1 EU/µg. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction. PuroSPIN™ buffer formulations produce highly purified plasmid DNA product for downstream applications, such as transfection, enzymatic digestion, or PCR.
 
Features:
Purify endotoxin-free plasmid DNA from small volumes of bacterial culture
Extract up to 15 µg of highly pure plasmid DNA with endotoxin levels <0.1 EU/µg
Simply extraction process with no need for phenol or chloroform
Optimized buffer formulations to ensure efficient removal of RNA from plasmid samples
Efficient removal of genomic DNA, protein, salt, and other contaminants
High quality extracted plasmid DNA can be used for a range of downstream applications such as cloning, PCR, restriction enzyme digest, sequencing transformation, or transfection
 
SKU List:
NKE111-50, NKE111-250