PuroSPIN™ Gel Extraction Kit #NK107-50, #NK107-250
PuroSPIN™ Gel Extraction Kit is designed to provide simple and easy-to-use method for extraction and purification of DNA samples (100 bp − 10 kbp) from agarose and PAGE gels. The purification technology is based on dissolving the gel embedded DNA in a buffer solution, followed by nucleic acid binding to silica spin-columns, which allows efficient removal of gel residues and other contaminants. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction. PuroSPIN™ buffer formulations produce highly purified DNA product for downstream applications, such as PCR, restriction digestion, ligation, labeling, or sequencing.
PuroSPIN™ columns can bind up to 200 μg of DNA product.
Features:
- Rapid purification of DNA fragments from standard agarose gels run in either TAE or TBE buffer
- Simple extraction process with no need for phenol or chloroform
- Efficient removal of dNTPs, primers, enzymes, and salts from PCR reaction mixtures and gel mixtures
- High quality extracted DNA can be used for a range of downstream applications such as sequencing, ligation, transformation, transfection, Southern blotting, qPCR, or microarray analysis
Citations:
- Stilwell, Justin M., et al. "Necroulcerative dermatitis associated with Myxobolus dermatoulcerans n. sp.(Cnidaria: Myxobolidae) in red-bellied piranha, Pygocentrus nattereri Kner (Characiformes: Serrasalmidae), from Peru." Systematic Parasitology 97.6 (2020): 649-659.
- Hawke, John P., et al. "Streptococcus dysgalactiae: A Pathogen of Feral Populations of Silver Carp from a Fish Kill Event." Journal of Aquatic Animal Health 33.4 (2021): 231-242.
- Coates, Ethan Robin. "Assessment and Characterization of Various Gene-editing Platforms for Brassica napus (Canola) using TRANSPARENT TESTA 8 (TT8) as the Target Gene." Assessment 2023 (2023): 12-21.
- Roth, S. A. (2024). Developing base-editing for the improvement of agronomic traits in field peas (Pisum sativum) using glyphosate resistance as a target (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.
- Bennett, Hannah M., et al. "Vibrio harveyi in a Caribbean spiny lobster (Panulirus argus) with hepatopancreas necrosis." Veterinary Pathology 60.5 (2023): 618-623.
SKU List: NK107-50, NK107-250
Product Manuals
Materials Safety Data Sheets (MSDS)
Product Specifications
Recovered DNA Size 70 bp – 10 kb DNA Recovery 70 – 80 % Column Binding Capacity 200 µg Column Reservoir Capacity 800 µL Kit Comparison
Table 1: Extraction of 1500 bp dsDNA strand from 1% agarose gel
PuroSPIN Gel Extraction Kit
Comp. Q Gel Extraction Kit
Comp. B Gel Extraction Kit
Yield (μg)
1.9 (±0.2)
1.9 (±0.1)
2 (±0.4)
Recovery (%) 73 (±7) 71 (±3) 76 (±14) 260/280 1.8 (±0.1) 1.8 (±0.1) 1.7 (±0.1)
260/230 1.4 (±0.2)
0.5 (±0.6)
0.8 (±0.4) * Data based on 3 head-to-head replicates.
Product Data Sheet
