PuroSPIN™ PCR Product Purification Kit #NK105-50, #NK105-250
- PuroSPIN™ PCR Purification Kit is designed to provide simple and easy-to-use method for purification of PCR DNA products (70 bp − 10 kb) from enzymatic reactions.
- High concentration, yield and purity product:
- The purification technology is based on nucleic acid binding to silica spin-columns, which allows efficient removal of protein, salt and other contaminants. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction.
- PuroSPIN™ buffer formulations produce highly purified DNA product for downstream applications, such as sequencing, ligation, transfection, Southern blotting, qPCR, or microarray analysis.
- DNA purified by PuroSPIN™ PCR Product Purificaiton Kit shows equal or higher PCR product yield and purity (as determined by the 260/280 and 260/230 ratios) when compared to leading competitors in the field
- see head-to-head comparison data in the "Competitor Kit Comparison" section below.
SKU List: NK105-50, NK105-250
Product Manuals
Materials Safety Data Sheets (MSDS)
Product Specifications
Kit Type: PCR Product Purification Kit Format: Silica Spin Columns Kit Scale: MINI PCR Product Size: 70 bp − 10 kb Purified Product Yield: 1 - 5 µg Purified Product Concentration: 10 - 25 ng / µL Purified Product 260 / 280 Ratio: 1.7 - 3.0 Purified Product 260 / 230 Ratio: 1.8 - 3.3 Competitor Kit Comparison
Data shown below compared purificaiton efficiency and purity of the product produced by the PuroSPIN™ PCR Product Purificaitn Kit and leading competitor kits. For these experiments PCR product was generated using standard 100 μL scale reaction. 10 μL of this PCR product was used for the tests. 100 bp, 150 bp, and 1500 bp PCR products were tested.
Table 1: Comparison of PCR product purification kits: 100 bp product
PuroSPIN PCR Kit
Comp. Q PCR Kit Comp. B PCR Kit Yield (μg) 1.4 (±0.2) 1.0 (±0.1) 0.8 (±0.3) 260/280 3.0 (±0.6) 2.2 (±0.1) 2.7 (±1.8) 260/230 3.3 (±0.4) 0.2 (±0.1) 0.9 (±0.4) * Data based on 3 replicates. Numbers in brackets are standard deviations.
Table 2: Comparison of PCR product purification kits: 150 bp product
PuroSPIN PCR Kit
Comp. Q PCR Kit Comp. B PCR Kit Yield (μg) 1.3 (±0.1) 1.1 (±0.1) 1.0 (±0.1) 260/280 3.0 (±0.4) 2.3 (±0.3) 1.8 (±0.2) 260/230 2.9 (±0.3) 0.1 (±0.1) 0.8 (±0.2) * Data based on 3 replicates. Numbers in brackets are standard deviations.
Table 3: Comparison of PCR product purification kits: 1500 bp product
PuroSPIN PCR Kit
Comp. Q PCR Kit Comp. B PCR Kit Yield (μg) 3.8 (±0.1) 2.6 (±0.3) 4.2 (±0.1) 260/280 1.7 (±0.1) 1.9 (±0.1) 1.8 (±0.1) 260/230 1.8 (±0.1) 0.2 (±0.1) 1.0 (±0.9) * Data based on 2 replicates. Numbers in brackets are standard deviations.
Product Data Sheet
