PuroSPIN™ Plasmid MAXIprep Plus Kit #NK213-10, #NK213-30
PuroSPIN™ Plasmid MAXIprep Plus Kit is designed to provide simple and easy-to-use method for extraction and purification of plasmid DNA from cultured bacterial cells. The purification technology is based on plasmid binding to silica spin-columns, which allows efficient removal of genomic DNA, protein, salt and other contaminants. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction. PuroSPIN™ buffer formulations produce highly purified plasmid DNA product for downstream applications, such as transfection, enzymatic digestion, or PCR.
Features:
- PuroSPIN™ Plasmid MAXIprep Plus Kit can be used to purify plasmid DNA from up to 300 mL of overnight bacterial culture
- Extaction of up to 500 µg of plasmid DNA product
- Plus kit includes a syringe filter that quickly and easily removes insoluble fraction from the plasmid preparation, removing the need for the 12,000 x g centrifugation step
- Purify plasmid DNA from large volumes of bacterial culture
- Rapid purification of plasmid DNA (< 60 minutes), compared to ion-exchange column formats (> 3 hours)
- Efficient removal of genomic DNA, protein, salt, and other contaminants
- High quality extracted plasmid DNA can be used for a range of downstream applications such as cloning, PCR, restriction enzyme digest, sequencing, transformation, or transfection
- Endotoxin-free formats are also available
SKU List:
NK213-10, NK213-30
Product Manuals
Product Data Sheet