PuroSPIN™ Total RNA Purification Plus Kit #NK251-50, #NK251-200

PuroSPIN™ Total RNA Purification Plus Kit is designed to purify total RNA from bacteria, mammalian cells, yeast, or animal tissue. It greatly simplifies the extraction process by incorporating a convenient easy-to-use extraction technology based on nucleic acid binding silica spin-columns.
In comparison to our standard Total RNA Purification Kit, the Plus kit adds an additional filtration step that removes the insoluble and non-digested fraction as well as insuring elimination of genomic DNA from the lysate before loading it onto the silica spin column. This prevents contaminating materials from binding to and blocking parts of the silica membrane, leading to an increased RNA yield and higher purity of the final product. This is particularly benficial when workign with large samples that contain a lot of starting material that needs to be digested.
In addition the kit includes on-column DNase I digestion step to further ensure removal of any contaminating DNA. 
The kit can also be used to perform RNA cleanup after DNase I or other enzymatic treatments. High quality RNA extracted with the PuroSPIN™ Total RNA Purification Plus Kit can be used for a range of downstream applications, such as reverse-transcription PCR or qPCR, northern blotting, microarray analysis.
 
PuroSPIN™ Total RNA Purification Plus Kit is capable of extracting RNA from soft animal tissue, such as liver, spleen, kidney, lung. For purification of RNA from fibrous tissue, such as skin, skeletal muscle, or heart, please use our PuroSPIN™ Fibrous Tissue RNA Purification Kit(#NK053-50, #NK053-250) or PuroSPIN™ Fibrous Tissue RNA Purification Plus Kit (#NK253-50, #NK253-200).
 
Features:
Extract total RNA from a variety of samples, with specialized protocols for each sample type
Simple extraction process with no need for phenol or chloroform
Includes a filtration and on-column/on-bead DNase I digestion steps to remove contaminating DNA
High quality extracted RNA can be used for a range of downstream applications such as cDNA library construction, microarray analyses, Northern blotting, qPCR, and RT-qPCR
 
Citations:
Szojka, Alexander RA, et al. "Mechano-hypoxia conditioning of engineered human meniscus." Frontiers in bioengineering and biotechnology 9 (2021).
Aissiou, A.K., Jha, S., Dhunnoo, K. et al. Transcriptomic response of bioengineered human cartilage to parabolic flight microgravity is sex-dependent. npj Microgravity 9, 5 (2023).
Ma, Z., Li, D.X., Chee, R.K.W. et al. Mechanical Unloading of Engineered Human Meniscus Models Under Simulated Microgravity: A Transcriptomic Study. Sci Data 9, 736 (2022).
Lan, X., Ma, Z., Kunze, M. et al. The Effect of Crosslinking Density on Nasal Chondrocytes’ Redifferentiation. Ann Biomed Eng (2023). https://doi.org/10.1007/s10439-023-03184-3
Li DX, Ma Z, Szojka AR, Lan X, Kunze M, Mulet-Sierra A, Westover L, Adesida AB. Non-hypertrophic chondrogenesis of mesenchymal stem cells through mechano-hypoxia programing. J Tissue Eng. 2023 May 16;14:20417314231172574. doi: 10.1177/20417314231172574. PMID: 37216035; PMCID: PMC10192798.
 
SKU List: NK251-5, NK251-50, NK251-200