Streptavidin Coated Magnetic Beads, 500 nm Diameter
500 nm diameter magnetic beads with Streptavdin protein functionalized surface.
Features
- High binding capacity of Biotin-conjugated molecules:
- 2,000 to 4,000 pmole of biotin / mg of magnetic beads
- 15-20 µg botinylated antibody / mg of magnetic beads
- Rapid and efficient biomolecule purification from complex samples.
- Fast response to a magnet.
- High monodispersity.
- Stable in high salt conditions.
Applications
- Magnetic separation of Biotinylated cells, DNA, proteins, antibodies, and small ligands from complex samples. The streptavidin-coated magnetic beads are simply added to Biotinylated molecules for binding. The samples are transferred into a magnetic rack for easy removal of unbound residue.
Citations:
- J. H. Molinski, S. Parwal and J. X. J. Zhang, "Laser-Manufactured Magnetic Microchips for Exosome Isolation and Patterning," 2024 IEEE 37th International Conference on Micro Electro Mechanical Systems (MEMS), Austin, TX, USA, 2024, pp. 336-339, doi: 10.1109/MEMS58180.2024.10439482.
- Molinski, John H., Siddhant Parwal, and John XJ Zhang. "Laser‐Patterning of Micromagnets for Immuno‐Magnetophoretic Exosome Capture." Small Methods (2024): 2400388.
Detailed product information
MSDS
Protocols
Protocol A: Immobilization of proteins / antibodies
- Vortex PuroMAG™ magnetic beads before use to fully resuspend the beads.
- Transfer the desired volume of beads into a new 1.5 mL microcentrifuge tube.
- Add biotinylated protein / antibody in 1X PBS to the tube. Incubate for 30 min at room temperature using gentle rotation.
- Place the beads on the magnetic rack and incubate for 2 min. Remove the supernatant.
- Remove the tube from the rack, add 1 mL of Wash Buffer PR and mix well. Place the tube on the magnetic rack and incubate for 2 min. Remove the supernatant.
- Repeat washing Step 5 two more times.
- Resuspend in the desired amount of 1X PBS.
NOTE: If a significant amount of non-specific adsorption of proteins / antibodies to the beads is observed, pre-wash the beads in Wash Buffer PR before performing protein / antibody immobilization:
- Place the beads on the magnetic rack and incubate for 2 min. Remove the supernatant. Resuspend in the volume of Wash Buffer PR equal to the volume of stock beads used.
Protocol B: Immobilization of nucleic acids
- Vortex PuroMAG™ magnetic beads before use to fully resuspend the beads.
- Transfer the desired volume of beads into a new 1.5 mL microcentrifuge tube.
- Place the tube on the magnetic rack and incubate for 2 min. Discard the supernatant.
- Add the same volume of Wash Buffer NA, 2X as the volume of stock beads used.
- Add an equal volume of biotinylated DNA or RNA nucleic acid resuspended in ultra-pure water.
- Incubate for 15 min at room temperature using gentle rotation.
- Place the beads on the magnetic rack and incubate for 2 min. Remove the supernatant.
- Remove the tube from the rack, add 1 mL of Wash Buffer NA, 1X and mix well. Place the tubes on the magnetic rack and incubate for 2 min. Remove the supernatant.
- Repeat washing Step 8 two more times.
- Resuspend the washed beads in the desired amount of 1X PBS or alternative buffer appropriate for the downstream application.
Protocol C: Releasing Immobilized Biotinylated Proteins / Antibodies
- Boil the samples for 5 min in 0.1% SDS. This will denature the proteins, including streptavidin, and will release biotinylated molecules into the solution.
Protocol D: Releasing Immobilized Biotinylated DNA
- Incubate the beads for 5 min at 65°C (or 2 min at 90°C) in 10 mM EDTA with 95% formamide.
$245.00Price
