Streptavidin, Fluorescent Dye Label

Recombinant Streptavidin protein that has been covalently labeled with a fluorescent dye.
 
Available as a lyophilized powder or 2 mg/mL solution
 
The following fluorescence dye labels are available:
AFDye 488
AFDye 555
AFDye 647
IR750
 
NOTE 1: AFDye 488 (similar to FITC), AFDye 555 (similar to Cy3), AFDye 647 (similar to Cy5) are analogs of AlexaFluor® 488, AlexaFluor® 555, and AlexaFluor® 647 dyes, respectively.
NOTE 2: IR750 dye is ithe analog of the IRDye® 750 dye.
 
Streptavidin is a homo-tetrameric protein found in the culture broth of Streptomyces avidinii. Similar to avidin, one mole of streptavidin can bind 4 moles of biotin with a high affinity. Streptavidin lacks carbohydrate side chains present on avidin and has a neutral isoelectric point, which reduces nonspecific binding compared to avidin. Streptavidin is extensively used for various biological applications, including ELISA, IHC, TRFIA, quantification of PCR product, isolation of single-stranded nucleotide, purification of biomolecule, and monoclonal antibody production as well.
 
Compared to native streptavidin, our recombinant streptavidin has improved stability and solubility through the removal of activity-unrelated amino acid sequences.
 
Applications
Western blots
Flow cytometry
Imaging and microscopy
Microplate assays
Immunoprecipitation of antibodies and antibody / antigen complexes
ELISA
Purification of biomolecules
 
Storage and Handling
3 years at -20˚C.  After reconstitution, aliquot and store at -20˚C.  Avoid repeated freeze/thaw cycles.
 
Intended Use
Luna Nanotech Streptavidin is provided for research laboratory or bioprocessing purposes only. Not for use in diagnostic or therapeutic human or animal applications.
 
Formulation
Lyophilized without additives and carrier free.
 
Purity
≥ 95% by SDS PAGE and HPLC analysis.
 
Source
Escherichia coli
 
Storage
Store, frozen at -20°C for up to 3 years. After reconstitution in distilled water or buffer, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.