Streptavidin, Recombinant, Lyophilized

Streptavidin is a homo-tetrameric protein found in the culture broth of Streptomyces avidinii. Similar to avidin, one mole of streptavidin can bind 4 moles of biotin with a high affinity. Streptavidin lacks carbohydrate side chains present on avidin and has a neutral isoelectric point, which reduces nonspecific binding compared to avidin. Streptavidin is extensively used for various biological applications, including ELISA, IHC, TRFIA, quantification of PCR product, isolation of single-stranded nucleotide, purification of biomolecule, and monoclonal antibody production as well.
 
Compared to native streptavidin, our recombinant streptavidin has improved stability and solubility through the removal of activity-unrelated amino acid sequences.
 
Applications
Immunoprecipitation of antibodies and antibody / antigen complexes
Western blotting
Immunohistochemistry
ELISA
Purification of biomolecules
 
Storage and Handling
3 years at -20˚C.  After reconstitution, aliquot and store at -20˚C.  Avoid repeated freeze/thaw cycles.
 
Intended Use
Luna Nanotech Streptavidin is provided for research laboratory or bioprocessing purposes only. Not for use in diagnostic or therapeutic human or animal applications.
 
Formulation
Lyophilized without additives and carrier free.
 
Purity
≥ 95% by SDS PAGE and HPLC analysis.
 
Source
Escherichia coli
 
Storage
tore, frozen at -20°C for up to 3 years. After reconstitution in distilled water or buffer, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.